Figure 3
From: Genetically driven brain serotonin deficiency facilitates panic-like escape behavior in mice
Inhibitory currents in the basolateral nucleus of the amygdala of Tph2-deficient mice. Electrophysiological investigation of GABAergic synapse function in vitro of naive Tph2−/−, Tph2+/− and Tph2+/+ mice. (a) Upper panel: mean frequency (left) and amplitude (right) of spontaneous inhibitory postsynaptic currents (sIPSCs) in Tph2−/− (n=21/3), Tph2+/− (n=24/4) and Tph2+/+ (n=19/3) mice with example traces shown in the lower panel. (b) Upper panel: mean frequency and amplitude of miniature inhibitory postsynaptic currents (mIPSCs) in Tph2−/− (n=18/4), Tph2+/− (n=18/3) and Tph2+/+ (n=16/3) mice with example traces shown in the lower panel. (c) Scheme of recording indicating the stimulating electrode in the lateral nucleus (LA) and the patch-clamp pipette in the basolateral nucleus of the amygdala (BL). (d) Mean amplitude of evoked inhibitory postsynaptic currents (eIPSCs) recorded at different stimulus intensities in pyramidal neurons from Tph2−/−, Tph2+/− and Tph2+/+ mice. Note significant reduction of eIPSCs at all tested stimulation intensities in Tph2−/− (n=14/4) and Tph2+/− (n=13/3) compared to wild types (n=12/3) as well as paired-pulse ratio (e). Data are shown as means+s.e.m. *P<0.05, **P<0.01 and ***P<0.001.
