Figure 2

Tooth eruption is delayed in Adnp^+/− mice. Two- and five-day-old mouse pup heads were subjected to micro-computed tomography (μCT). (a–c) A trend of delayed tooth eruption in 2-day-old Adnp^+/− pups compared to Adnp^+/+ pups. (a) Dental sac (also defined as the dental follicle contains the developing tooth and its odontogenic organ, n=3 mice per group). (b) Tooth buds (the tooth bud contains the dental follicle/sac, the enamel organ and the dental papilla, n=3 mice per group). (c) Picture. (d–h) Delayed tooth eruption in 5-day-old Adnp^+/− pups as measured both by the size of the dental sac and the tooth buds of molars (d, e), respectively, n=3 mice per group, *P<0.05; **P<0.01, Student's t-test) and of incisors (f, g), respectively, n=3 mice per group, *P<0.05; ****P<0.0001, Student's t-test). A picture of representative molars at 5 days of age is shown comparing Adnp^+/+ mice to Adnp^+/− mice (h). (i) Allen Atlas analysis identified ADNP expression in the jaw area. Pictures obtained at embryonic age E13.5 and E15.5 are shown with a clear enrichment of Adnp expression in the jaw area (red, in situ hybridizations). ADNP, activity-dependent neuroprotective protein.