Figure 4
From: Adult hippocampal neuroplasticity triggers susceptibility to recurrent depression
Imipramine enhances dentate gyrus (DG) neuronal arborization, whereas fluoxetine promotes an atrophy of granule neurons after recurrent stress. Dendritic length analysis (a) and neuronal organization (b) of DG granule neurons showed a dendritic shrinkage promoted by fluoxetine treatment, whereas imipramine induced an enlargement of the neuronal arborization after stress re-exposure. (c) Representative three-dimensional (3D) morphometric reconstruction of DG granule neurons of each experimental group. (d and e) The relative gene expression levels of remodeling genes, NCAM and SYN1, corroborates the neuronal remodeling promoted by imipramine treatment. Scale bars represent 50 μm. *Denotes the effect of unpredictable chronic mild stress (uCMS) analyzed by Student’s t-test. #Denotes the effect of ADs, by comparison of treatment and SAL animals; and ‡denotes differences between ADs, analyzed by one-way analysis of variance (ANOVA). ANOVA repeated measures was used to analyze Sholl analysis. Data are represented as mean±s.e.m. #, ‡P⩽0.05, **, ‡‡P⩽0.01, ***, ###, ‡‡‡P⩽0.001; n=± 4 animals per group. AD, antidepressant; CTRL, non-stressed animals; FLX, animals repeatedly exposed to uCMS and treated with fluoxetine; IMIP, animals repeatedly exposed to uCMS and treated with imipramine; SAL, animals repeatedly exposed to uCMS and non-treated.
