Figure 4
From: Adenosine A2A receptor and ERK-driven impulsivity potentiates hippocampal neuroblast proliferation
Mice lacking ENT1 display aberrant impulsivity during DRL-conditioning and exhibit dampened dorsal hippocampus (dHip) A2AR expression and lowered ERK1/2 phosphorylation. (a) Western blot and densitometry quantitation show significantly decreased expression of A2AR in the dHip of behavior-naive ENT1–/– mice versus WT mice. n=11 mice per genotype. *P<0.05 by Student’s t-test. (b) Behavior naive ENT1–/– mice have decreased pERK2 in the dHip relative to WT mice. n=3 per genotype. *P<0.05 by Student’s t-test. (c) There were no differences in pERK2 in the nucleus accumbens (NAc) of naive WT and ENT1–/– mice. n=4 mice per genotype. (d) Schematic of experimental design for testing impulsivity during Pavlovian-conditioning in ENT1–/– mice versus WT mice. (e) ENT1–/– mice display significantly higher magazine entries for sucrose reward during conditioning. (f) Relative to WT mice, ENT1–/– mice display faster reaction times to retrieve sucrose reward in response to CS+. All data are reported as mean±s.e.m. RM two-way ANOVA, #P<0.05 main effect of genotype; *P<0.05 by Tukey’s post hoc multiple comparisons versus WT mice. (e, f n=13–15 per genotype). DRL, differential reward of low rate; RM, repeated measures; WT, wild-type.
