Figure 5

Expression of markers for cell proliferation and neuroblast development in the hippocampal dentate gyrus (DG) is affected by ENT1 deletion and A_2AR inhibition-mediated impulsivity. (a) Representative confocal microscopy images and stereological quantification indicate that relative to WT, naive ENT1^–/– mice have decreased numbers of BrdU⁺-labeled cells (green). DAPI (blue). Scale bar, 100 μm. (b) Relative to WT, naive ENT1^–/– mice have decreased numbers of MCM2⁺ (green) DCX⁺ (red)-labeled cells. DAPI (blue). Scale bar, 100 μm. All data are reported as mean±s.e.m. (a, b) n=5 brain sections per brain and n=4–5 mice per genotype. *P<0.01 by unpaired two-tailed Student’s t-test. (c) Analysis of BrdU⁺-labeled cells (green) in 5-CSRTT-tested mice indicate overall higher numbers of BrdU⁺ cells in mice administered ZM-241385 relative to vehicle. DAPI (blue). Scale bars, 100 μm. (d) Representative confocal microscopy images and stereological quantification indicate that in comparison to vehicle, mice administered ZM-241385 that displayed increased impulsivity during the 5-CSRTT exhibited higher numbers of BrdU⁺ (green) DCX⁺ (red) co-labeled cells. Thick arrows indicate representative individual BrdU⁺, DCX⁺ and DAPI co-labeled cells (see insets). Thin arrows indicate the subgranular zone (SGZ) or the DG of the hippocampus. Scale bars, 100 μm. (c, d) n=5–6 sections per brain and n=5–6 mice per treatment. *P<0.001 by unpaired two-tailed Student’s t-test. All data are reported as mean±s.e.m. 5-CSRTT, 5-choice serial reaction time task; DRL, differential reward of low rate; GCL, granular cell layer; H, hilus; ML, molecular layer; RM, repeated measures; WT, wild-type.