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This protocol details the fabrication and application of a 3D-printed device for facilitating lateral tail-vein injection in mice. The tail vein–illuminating ring comprises a light-emitting diode (LED) lamp box on the side; once the tail has been illuminated by the LED, the vein is more clearly visible along the lateral side of the mouse tail.
A new rat model of brainstem ischemia is described. Selective ligation of four points of the lower basilar artery causes a localized brainstem ischemic lesion in adult rats, resulting in hemiparesis, as well as abnormal posture, body balance and locomotion.
