Volume 21

Spatial proteomics is our pick for Method of the Year 2024. The cover illustrates cellular neighborhoods within tumors, revealing the diverse and complex microenvironments that shape tumor biology.

See Editorial

An image of a section of small intestine from a mouse won fourth place in the Nikon Small World 2024 Photomicrography competition.

See Editorial

This month, Nature Methods celebrates its 20th anniversary with a special feature.

See Editorial

Artistic representation of the sectioning step in a focused ion beam-based sample preparation technique, Serial Lift-Out. A block of vitreously frozen biological material (here, a C. elegans L1 larva embedded in buffer) is attached to a micromanipulator needle and transferred to a rectangular-mesh copper electron microscopy grid to be serially sectioned.

See Article

Advanced artificial intelligence (AI)-based methods are having a transformative impact on biological research, as explored in this special issue.

See Editorial

c-Fos+ neuronal mapping of whole mouse brains using DELiVR reveals cancer-induced brain activity changes.

See Kaltenecker et al.

Transverse view of a whole-brain tract-density reconstruction of white matter pathways in the chimpanzee brain. Color indicates tissue orientation and brightness encodes density of reconstructed fiber streamlines.

See Eichner et al.

A scanning electron microscope image captures the dynamic interplay between a CD19-hexapod biomimetic antigen-presenting structure and an anti-CD19 CAR-T cell.

See Huang et al.

Peptide sequencing by nanopore: a crow drops grapes into a pitcher with a narrow neck, representing the cleavage of a peptide into amino acids and their subsequent detection by a modified nanopore.

See Zhang et al.

Autonomously glowing Arabidopsis thaliana plants express an improved version of the fungal bioluminescence pathway.

See Shakhova et al.

Smart lattice light-sheet microscopy captures rare cellular events. The image shows immune synapses formed between cytotoxic T lymphocytes (cyan) and tumor cells (magenta) within a population of cultured cells. Cytotoxic granules are shown in yellow.

See Shi et al.

DeepMainmast builds structures of protein complexes from cryo-electron microscopy maps. It uses deep learning to identify key atom positions in the density, which are then connected to build fragment structures. Fragments are combined into a full structure, which is refined to atomic detail.

See Terashi et al.