The interaction between the different molecular mechanisms that regulate changes in embryonic stem cell (ESC) fate is not fully understood. A dynamic systems-level study of cell fate change in murine ESCs following a well-defined perturbation is now presented; the data demonstrate how a single genetic perturbation leads to widespread changes in several molecular regulatory layers, and provide a dynamic view of information flow in the epigenome, transcriptome and proteome.
- Rong Lu
- Florian Markowetz
- Ihor R. Lemischka