The nuclear spin-lattice relaxation rate can be used to probe binding of a rapidly moving small ligand to a more slowly moving larger protein, however, the measurements are challenging at high magnetic fields. Here, the authors report low-field relaxation as a sensitive contrast mechanism for detecting ligand binding, using a hyperpolarization experiment to detect ligand signals with high repeatability at concentrations as low as 14 μM.
- Pooja Narwal
- Nils Lorz
- Benno Meier
