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Low pH structure of heliorhodopsin reveals chloride binding site and intramolecular signaling pathway

Jessica E. Besaw
Jörg Reichenwallner
Oliver P. Ernst
ResearchOpen Access17 Aug 2022
Scientific Reports

Volume: 12, P: 1-16
High-throughput in situ X-ray screening of and data collection from protein crystals at room temperature and under cryogenic conditions

This protocol describes procedures for the high-throughput in situ X-ray screening of and data collection from soluble and membrane-protein crystals at room temperature and under cryogenic conditions using Mylar-based film sandwich plates.

Jana Broecker
Takefumi Morizumi
Oliver P Ernst
Protocols04 Jan 2018
Nature Protocols

Volume: 13, P: 260-292

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Low pH structure of heliorhodopsin reveals chloride binding site and intramolecular signaling pathway

High-throughput in situ X-ray screening of and data collection from protein crystals at room temperature and under cryogenic conditions

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