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Communications Biology (2)

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Dynamics of agonist-evoked opioid receptor activation revealed by FRET- and BRET-based opioid receptor conformation sensors

The study describes the development of FRET- and BRET-based receptor conformations sensors for the opioid receptor family. These sensors further revealed strongly agonist-dependent activation kinetics for the µ opioid receptor.

Sina B. Kirchhofer
Claudia Kurz
Moritz Bünemann
ResearchOpen Access08 Feb 2025
Communications Biology

Volume: 8, P: 1-11
Interaction kinetics between p115-RhoGEF and Gα₁₃ are determined by unique molecular interactions affecting agonist sensitivity

The three RH-RhoGEFs differ in their sensitivity of activation by G proteins. For p115-RhoGEF, a specific molecular interaction with Gα₁₃ is responsible for faster kinetics and lower sensitivity.

Fabian Redlin
Anna-Lena Krett
Moritz Bünemann
ResearchOpen Access24 Nov 2022
Communications Biology

Volume: 5, P: 1-10

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Dynamics of agonist-evoked opioid receptor activation revealed by FRET- and BRET-based opioid receptor conformation sensors

Interaction kinetics between p115-RhoGEF and Gα13 are determined by unique molecular interactions affecting agonist sensitivity

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Interaction kinetics between p115-RhoGEF and Gα₁₃ are determined by unique molecular interactions affecting agonist sensitivity