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Showing 1–13 of 13 results
Advanced filters: Author: Antoine Royant Clear advanced filters

  • Cyan variants of green fluorescent protein (CFPs) are widely used as donors in FRET experiments. Here, a new CFP, mTurquoise2, is developed, which displays a high-fluorescence quantum yield and a long mono-exponential fluorescence lifetime.

    • Joachim Goedhart
    • David von Stetten
    • Antoine Royant
    ResearchOpen Access
    Nature Communications
    Volume: 3, P: 1-9

  • Using structural, functional and spectroscopic investigations of a bacterial inward proton-pumping rhodopsin, Kovalev et al. reveal its molecular mechanism and show how proton wires mediate ion selectivity and direct proton transport through cell membrane.

    • Kirill Kovalev
    • Fedor Tsybrov
    • Valentin Gordeliy
    Research
    Nature Structural & Molecular Biology
    Volume: 30, P: 970-979

  • The bacterial thymidylate synthase ThyX catalyzes the reductive methylation of deoxyuridylate (dUMP) into deoxythymidylate (dTMP) and requires both folate and flavin for activity. Here, the authors combine biochemical experiments, spectroscopic measurements and flavin synthesis chemistry to show that formaldehyde (CH2O) can replace the natural methylene donor of ThyX in a CH2O-shunt reaction, yielding a carbinolamine intermediate with the reduced flavin coenzyme, and they present the crystal structure of this intermediate.

    • Charles Bou-Nader
    • Frederick W. Stull
    • Djemel Hamdane
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-8

  • The Na+-pumping KR2 rhodopsin from Krokinobacter eikastus is a light-driven non-proton cation pump whose mechanism of pumping remains to be understood. Here authors solved crystal structures of the O-intermediate state of the pentameric form of KR2 and its D116N and H30A mutants, which sheds light on the mechanism of non-proton cation light-driven pumping.

    • Kirill Kovalev
    • Roman Astashkin
    • Valentin Gordeliy
    ResearchOpen Access
    Nature Communications
    Volume: 11, P: 1-11

  • Infrared fluorescent proteins offer advantages for deep in vivo imaging thanks to the tissue-penetrating properties of infrared light. Here, Yu et al. design a monomeric infrared fluorescent protein that, when combined with expression of haeme oxygenase in cells, shows improved performance for in vivoimaging of neurons and brain tumours.

    • Dan Yu
    • William Clay Gustafson
    • Xiaokun Shu
    Research
    Nature Communications
    Volume: 5, P: 1-7

  • A reduction reaction is usually equated with an electron transfer reaction. Now, ultrafast time-resolved serial femtosecond X-ray crystallography has enabled the visualization of the stepwise structural changes that occur after electron transfers have been observed in the light-triggered reduction of flavin adenine dinucleotide catalysed by DNA photolyase.

    • Manuel Maestre-Reyna
    • Cheng-Han Yang
    • Ming-Daw Tsai
    Research
    Nature Chemistry
    Volume: 14, P: 677-685

  • Time-resolved serial femtosecond crystallography is used to reveal the structural changes that stabilize the charge-separation steps of electron-transfer reactions in the photosynthetic reaction centre of Blastochloris viridis on a timescale of picoseconds.

    • Robert Dods
    • Petra Båth
    • Richard Neutze
    Research
    Nature
    Volume: 589, P: 310-314

  • Crystallographic snapshots illustrate the catalytic cycle and illuminate the mechanism by which the enzyme Pdx1 shuttles intermediates between lysine residues in its two active sites during the biosynthesis of pyridoxal 5′-phosphate.

    • Matthew J Rodrigues
    • Volker Windeisen
    • Ivo Tews
    Research
    Nature Chemical Biology
    Volume: 13, P: 290-294

  • Two mScarlet variants with high brightness and fast maturation times have been evolved. These variants behave favorably as fusion tags and Förster resonance energy transfer acceptors.

    • Theodorus W. J. Gadella Jr.
    • Laura van Weeren
    • Antoine Royant
    Research
    Nature Methods
    Volume: 20, P: 541-545