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Author Correction: Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors

Beau R. Webber
Cara-lin Lonetree
Branden S. Moriarity
Amendments and CorrectionsOpen Access06 Dec 2019
Nature Communications

Volume: 10, P: 1
CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells

Base editors can inactivate splice sites or introduce stop codons into a gene sequence. Here the authors present SpliceR to design, rank, and test sgRNAs for efficient gene disruption in T cells.

Mitchell G. Kluesner
Walker S. Lahr
Branden S. Moriarity
ResearchOpen Access23 Apr 2021
Nature Communications

Volume: 12, P: 1-12
Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors

Multiplexed genome engineering with Cas9 can increase efficiency but also the risk of unintended alterations. Here the authors demonstrate the use of multiplexed base editors on primary T cells with reduced translocation frequency.

Beau R. Webber
Cara-lin Lonetree
Branden S. Moriarity
ResearchOpen Access19 Nov 2019
Nature Communications

Volume: 10, P: 1-10
Germline T cell receptor exchange results in physiological T cell development and function

The currently available transgenic T cell receptor (TCR) models represent high affinity antigen-TCR interactions. Authors here present an alternative approach to target an exogenous TCR into the physiological Trac locus in the germline of mice, which uncovers that the natural genomic context for TCRs can enhance the antigen sensitivity of lower affinity TCRs and enables the physiologic range of antigen-TCR interaction and a gene dosage dependent mechanism of central tolerance.

Meagan R. Rollins
Jackson F. Raynor
Ingunn M. Stromnes
ResearchOpen Access01 Feb 2023
Nature Communications

Volume: 14, P: 1-19
Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair

The targeted integration of large DNA payloads into primary human T cells can be efficiently achieved non-virally by leveraging Cas9-based editing and the DNA-repair pathway homology-mediated end joining.

Beau R. Webber
Matthew J. Johnson
Branden S. Moriarity
Research13 Dec 2023
Nature Biomedical Engineering

Volume: 8, P: 1553-1570
A pig with functional human immunity

Genetically modified immunodeficient pigs support the development of functional human B and T cells and represent a preclinical model for investigating human haematopoiesis and a bioreactor for large-scale production of human immune cells for diverse therapies.

Christopher K. Tuggle
Crystal L. Loving
Beau R. Webber
News & Views11 Jun 2025
Nature Biomedical Engineering

P: 1-2
Ablation of FAS confers allogeneic CD3^– CAR T cells with resistance to rejection by T cells and natural killer cells

The ablation of FAS and CD3 in allogeneic chimeric antigen receptor T cells confers them with partial protection from rejection by T cells and natural killer cells in immunocompetent hosts.

Silvia Menegatti
Sheila Lopez-Cobo
Laurie Menger
Research18 Nov 2024
Nature Biomedical Engineering

Volume: 8, P: 1651-1664
Engineering T cells to enhance 3D migration through structurally and mechanically complex tumor microenvironments

The mechanics of the migration of T cells into tumours is an important aspect of tumour immunity. Here the authors engineer complex 3D environments to explore functions of microtubules and cell contractility as strategies to enhance T cell migration in tumour microenvironments.

Erdem D. Tabdanov
Nelson J. Rodríguez-Merced
Paolo P. Provenzano
ResearchOpen Access14 May 2021
Nature Communications

Volume: 12, P: 1-17
A BAFF ligand-based CAR-T cell targeting three receptors and multiple B cell cancers

Antigen escape represents a potential drawback of chimeric antigen receptor T cell (CAR-T) therapy targeting a single tumor-associated antigen. To reduce the risk of antigen escape, here the authors report the design and characterization of a BAFF ligand CAR-T that can recognize three different receptors (BAFF-R, BCMA and TACI), demonstrating in vitro and in vivo cytotoxicity against multiple B cell cancer models.

Derek P. Wong
Nand K. Roy
Reshmi Parameswaran
ResearchOpen Access11 Jan 2022
Nature Communications

Volume: 13, P: 1-17
Engineering of Primary Human B cells with CRISPR/Cas9 Targeted Nuclease

Matthew J. Johnson
Kanut Laoharawee
Branden S. Moriarity
ResearchOpen Access14 Aug 2018
Scientific Reports

Volume: 8, P: 1-9
CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa

Beau R Webber
Mark J Osborn
Jakub Tolar
ResearchOpen Access08 Dec 2016
npj Regenerative Medicine

Volume: 1, P: 1-11
Rapid generation of Col7a1^−/− mouse model of recessive dystrophic epidermolysis bullosa and partial rescue via immunosuppressive dermal mesenchymal stem cells

Beau R Webber
Kyle T O'Connor
Jakub Tolar
Research11 Sept 2017
Laboratory Investigation

Volume: 97, P: 1218-1224

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Author Correction: Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors

CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells

Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors

Germline T cell receptor exchange results in physiological T cell development and function

Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair

A pig with functional human immunity

Ablation of FAS confers allogeneic CD3^– CAR T cells with resistance to rejection by T cells and natural killer cells

Engineering T cells to enhance 3D migration through structurally and mechanically complex tumor microenvironments

A BAFF ligand-based CAR-T cell targeting three receptors and multiple B cell cancers

Engineering of Primary Human B cells with CRISPR/Cas9 Targeted Nuclease

CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa

Rapid generation of Col7a1^−/− mouse model of recessive dystrophic epidermolysis bullosa and partial rescue via immunosuppressive dermal mesenchymal stem cells

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