Class 2 CRISPR-Cas systems are widely used in genome editing. Joshua Young1, Stephen Gasior et al. now engineer the type I-E Cascade complex from S. thermophiles, optimize the system for expression and nuclear localization in Zea mays and show it compares favorably with Cas9 as a RNA-guided transcriptional activator.
- Joshua K. Young
- Stephen L. Gasior
- Rodolphe Barrangou