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A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells

Determining the trafficking of intracellular material is commonly done by colocalisation analysis using microscopy. Here the authors monitor trafficking of select cargo by measuring the conversion of quenched SNAP-tag substrates by subcellularly-localised SNAP-tag and detection by flow cytometry.

Laura I. FitzGerald
Luigi Aurelio
Angus P. R. Johnston
ResearchOpen Access08 Sept 2020
Nature Communications

Volume: 11, P: 1-13
Dynamic binding mode of a Synaptotagmin-1–SNARE complex in solution

The NMR-derived structural model of the dynamic interaction between synaptotagmin-1 and the SNARE complex contributes to addressing the longstanding problem of how synaptotagmin-1 triggers neurotransmitter release.

Kyle D Brewer
Taulant Bacaj
Josep Rizo
Research01 Jun 2015
Nature Structural & Molecular Biology

Volume: 22, P: 555-564
Probing the solution structure of the E. coli multidrug transporter MdfA using DEER distance measurements with nitroxide and Gd(III) spin labels

Eliane H. Yardeni
Thorsten Bahrenberg
Daniella Goldfarb
ResearchOpen Access29 Aug 2019
Scientific Reports

Volume: 9, P: 1-14

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