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Molecular beacon–enabled purification of living cells by targeting cell type–specific mRNAs

Molecular beacons are dual-labeled oligonucleotides that fluoresce in the presence of complementary mRNA. This protocol describes how to use them to purify specific cell populations by targeting mRNAs that are highly expressed only in the desired cell type.

Brian M Wile
Kiwon Ban
Gang Bao
Protocols18 Sept 2014
Nature Protocols

Volume: 9, P: 2411-2424

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