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The mechanism of RNA 5′ capping with NAD⁺, NADH and desphospho-CoA

RNA caps other than the 7-methylguanylate modification are generated by a distinct mechanism in which caps are added during, not after, transcription initiation through the use of non-canonical initiating nucleotides by RNA polymerases, a finding which has functional consequences.

Jeremy G. Bird
Yu Zhang
Bryce E. Nickels
Research06 Jul 2016
Nature

Volume: 535, P: 444-447
Detection of spacer precursors formed in vivo during primed CRISPR adaptation

Primed adaptation in the CRISPR-Cas system helps recognition of previously encountered sequence elements and promotes the formation of new memories. Here the authors characterized spacer precursors of type I-E and type I-F CRISPR-Cas system using in vivo models.

Anna A. Shiriaeva
Ekaterina Savitskaya
Ekaterina Semenova
ResearchOpen Access10 Oct 2019
Nature Communications

Volume: 10, P: 1-9
Quantitative analysis of transcription start site selection reveals control by DNA sequence, RNA polymerase II activity and NTP levels

Here, the authors use a massively parallel reporter assay RNA polymerase II massively systematic transcript end readout, to quantify factors that influence transcriptional start site selection in the genome of Saccharomyces cerevisiae to reveal patterns of dependence on DNA sequence, RNA polymerase II activity and nucleoside triphosphate abundance.

Yunye Zhu
Irina O. Vvedenskaya
Craig D. Kaplan
Research04 Jan 2024
Nature Structural & Molecular Biology

Volume: 31, P: 190-202
An RNA-seq method for defining endoribonuclease cleavage specificity identifies dual rRNA substrates for toxin MazF-mt3

MazF toxins inhibit bacterial growth by cleaving single-stranded RNA at specific sequences. Here, the authors describe a new genome-wide approach, MORE RNA-seq, for defining MazF cleavage specificity, and show that MazF-mt3 cleaves 23S and 16S ribosomal RNAs.

Jason M. Schifano
Irina O. Vvedenskaya
Nancy A Woychik
Research08 Apr 2014
Nature Communications

Volume: 5, P: 1-11
Growth-regulating Mycobacterium tuberculosis VapC-mt4 toxin is an isoacceptor-specific tRNase

Toxin–antitoxin systems of the Vap class regulate the growth of several bacterial pathogens including Mycobacterium tuberculosis. Here, the authors show that toxin VapC-mt4 arrests M. tuberculosis growth by specifically cleaving three tRNAs at a single site in their anticodon stem loop, leading to translation inhibition.

Jonathan W. Cruz
Jared D. Sharp
Nancy A. Woychik
Research09 Jul 2015
Nature Communications

Volume: 6, P: 1-12
A spatiotemporal molecular switch governs plant asymmetric cell division

Stomatal development requires asymmetric cell division and cell-fate determination, in which BASL is an essential regulator. The binding partners of BASL are now identified and characterized to reveal the molecular mechanism of cell-fate asymmetry in stomata.

Xiaoyu Guo
Chan Ho Park
Juan Dong
Research03 May 2021
Nature Plants

Volume: 7, P: 667-680
The σ⁷⁰ subunit of RNA polymerase mediates a promoter-proximal pause at the lac promoter

Bryce E Nickels
Jayanta Mukhopadhyay
Ann Hochschild
Research02 May 2004
Nature Structural & Molecular Biology

Volume: 11, P: 544-550

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The mechanism of RNA 5′ capping with NAD⁺, NADH and desphospho-CoA

Detection of spacer precursors formed in vivo during primed CRISPR adaptation

Quantitative analysis of transcription start site selection reveals control by DNA sequence, RNA polymerase II activity and NTP levels

An RNA-seq method for defining endoribonuclease cleavage specificity identifies dual rRNA substrates for toxin MazF-mt3

Growth-regulating Mycobacterium tuberculosis VapC-mt4 toxin is an isoacceptor-specific tRNase

A spatiotemporal molecular switch governs plant asymmetric cell division

The σ⁷⁰ subunit of RNA polymerase mediates a promoter-proximal pause at the lac promoter

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The mechanism of RNA 5′ capping with NAD+, NADH and desphospho-CoA

Detection of spacer precursors formed in vivo during primed CRISPR adaptation

Quantitative analysis of transcription start site selection reveals control by DNA sequence, RNA polymerase II activity and NTP levels

An RNA-seq method for defining endoribonuclease cleavage specificity identifies dual rRNA substrates for toxin MazF-mt3

Growth-regulating Mycobacterium tuberculosis VapC-mt4 toxin is an isoacceptor-specific tRNase

A spatiotemporal molecular switch governs plant asymmetric cell division

The σ70 subunit of RNA polymerase mediates a promoter-proximal pause at the lac promoter

Search

Quick links

The mechanism of RNA 5′ capping with NAD⁺, NADH and desphospho-CoA

The σ⁷⁰ subunit of RNA polymerase mediates a promoter-proximal pause at the lac promoter