NAD(P)H-dependent enzymes are generally assumed to use a one-step hydride transfer mechanism owing to a lack of evidence for alternative proposals. Spectrophotometric and NMR data now call this assumption into question, defining a covalent substrate-cofactor species that is catalytically competent in three unrelated enzymes.
- Raoul G Rosenthal
- Marc-Olivier Ebert
- Tobias J Erb
