Atomic Force Microscopy (AFM) provides precision imaging and force measurement offering great benefits in fields from nanotechnology to biology, but the accuracy in force measurements is still of order 10-25%. The authors present an alternative method, named Concurrent Atomic Force Spectroscopy, to study mechanical unfolding of proteins where AFM measurements are combined with Monte Carlo simulations to obtain how errors from the cantilever calibration propagate to errors in the forces measured, overcoming calibration uncertainty in relative mechanical measurements.
- Carolina Pimenta-Lopes
- Carmen Suay-Corredera
- Jorge Alegre-Cebollada
