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Showing 1–3 of 3 results
Advanced filters: Author: Cathleen Zeymer Clear advanced filters

  • Artificial metalloenzymes harness metal cofactors that are not naturally found in enzymes and embed these in specifically selected or designed protein scaffolds. In this Primer, Vornholt et al. describe the best practices for designing and engineering artificial metalloenzymes.

    • Tobias Vornholt
    • Florian Leiss-Maier
    • Thomas R. Ward
    Reviews
    Nature Reviews Methods Primers
    Volume: 4, P: 1-17

  • A chemoenzymatic method to site-specifically conjugate peptide and protein thioesters to folded proteins at lysine residues has been developed. The method uses a genetically encoded four-residue tag that is recognized by the E2 SUMO-conjugating enzyme Ubc9. This approach enables isopeptide formation with just Ubc9 in a programmable manner and obviates the need for E1 and E3 enzymes.

    • Raphael Hofmann
    • Gaku Akimoto
    • Jeffrey W. Bode
    Research
    Nature Chemistry
    Volume: 12, P: 1008-1015

  • The ER-resident Hsp70 BiP is regulated by NEF Bap. The interactions between BiP and Bap are now dissected using biochemistry, molecular modeling and smFRET approaches, revealing that Bap affects both domains of BiP, to coordinate release of substrate and nucleotide.

    • Mathias Rosam
    • Daniela Krader
    • Johannes Buchner
    Research
    Nature Structural & Molecular Biology
    Volume: 25, P: 90-100