BiP is an Hsp70 chaperone in the endoplasmic reticulum (ER) and is crucial for protein folding and quality control. Using single-molecule and ensemble FRET, the conformational cycle of BiP has now been defined. Movement of the lid domain of BiP allows this chaperone to discriminate between peptide and protein substrates.
- Moritz Marcinowski
- Matthias Höller
- Johannes Buchner
