A high-throughput precision oncology pipeline for pharmacotranscriptomic profiling using live-cell barcoding and single-cell RNA sequencing was developed. Using this approach revealed a drug resistance feedback loop in high-grade serous ovarian cancer, suggesting that combining phosphatidylinositol 3-OH kinase, protein kinase B and mammalian target of rapamycin inhibitors with epithelial growth factor receptor inhibitors may overcome resistance.
- Alice Dini
- Harlan Barker
- Daniela Ungureanu
