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Engineering of extracellular vesicles for efficient intracellular delivery of multimodal therapeutics including genome editors

Delivering functional proteins or protein complexes into cells continues to be a significant challenge. Here, the authors develop efficient systems by using engineered extracellular vesicles to deliver functional cargoes, including CRISPR/Cas9-ribonucleoproteins, both in vitro and in vivo.

Xiuming Liang
Dhanu Gupta
Samir EL Andaloussi
ResearchOpen Access29 Apr 2025
Nature Communications

Volume: 16, P: 1-18
Amelioration of systemic inflammation via the display of two different decoy protein receptors on extracellular vesicles

The loading of two different protein therapeutics onto extracellular vesicles can be optimized by genetically engineering the parent cells, as shown for extracellular vesicles displaying decoy receptors for two pro-inflammatory cytokines.

Dhanu Gupta
Oscar P. B. Wiklander
Samir El-Andaloussi
Research06 Oct 2021
Nature Biomedical Engineering

Volume: 5, P: 1084-1098
Publisher Correction: A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA

Olivier G. de Jong
Daniel E. Murphy
Pieter Vader
Amendments and CorrectionsOpen Access31 Mar 2020
Nature Communications

Volume: 11, P: 1
Identification of scaffold proteins for improved endogenous engineering of extracellular vesicles

Extracellular vesicles are naturally occurring nanoparticles that are gaining ground as delivery modalities for therapeutics. Here, the authors conducted a large-scale screening programme to identify potential scaffold proteins for cargo loading into extracellular vesicles.

Wenyi Zheng
Julia Rädler
Samir EL Andaloussi
ResearchOpen Access07 Aug 2023
Nature Communications

Volume: 14, P: 1-14
Phosphatidylserine-exposing extracellular vesicles in body fluids are an innate defence against apoptotic mimicry viral pathogens

Phosphatidylserine-exposing extracellular vesicles in body fluids can inhibit infection of viruses that use viral apoptotic mimicry for infection, but not viruses that use other entry mechanisms.

Rüdiger Groß
Hanna Reßin
Janis A. Müller
ResearchOpen Access25 Mar 2024
Nature Microbiology

Volume: 9, P: 905-921
Antibody-displaying extracellular vesicles for targeted cancer therapy

Extracellular vesicles decorated with an antibody-binding moiety specific for the fragment crystallizable domain can be used as a modular delivery system for targeted cancer therapy.

Oscar P. B. Wiklander
Doste R. Mamand
Samir EL Andaloussi
ResearchOpen Access20 May 2024
Nature Biomedical Engineering

Volume: 8, P: 1453-1468
A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA

Extracellular vesicles (EV) facilitate intercellular transfer of biological material including RNA, but the regulatory mechanisms for their formation and transfer are incompletely known. Here the authors develop a CRISPR-based reporting system to detect the transfer of guide RNAs and identify genes not previously linked to EV-mediated RNA delivery.

Olivier G. de Jong
Daniel E. Murphy
Pieter Vader
ResearchOpen Access28 Feb 2020
Nature Communications

Volume: 11, P: 1-13
Novel endosomolytic compounds enable highly potent delivery of antisense oligonucleotides

Two new endosomolytic small compounds increase delivery of splice-switching oligonucleotides by interfering with their trafficking to the LAMP1-positive endosomal compartments, inducing endosomal membrane rupture and concurrent oligonucleotide escape into the cytosol.

Jeremy P. Bost
Miina Ojansivu
Samir El Andaloussi
ResearchOpen Access01 Mar 2022
Communications Biology

Volume: 5, P: 1-14

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