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Photoswitching fingerprint analysis bypasses the 10-nm resolution barrier

Energy transfer between fluorophores is shown to impede SMLM at sub-10-nm spatial resolution. Time-resolved detection and photoswitching fingerprinting analysis are used to determine the number and separation of closely spaced fluorophores.

Dominic A. Helmerich
Gerti Beliu
Markus Sauer
ResearchOpen Access01 Aug 2022
Nature Methods

Volume: 19, P: 986-994
Photoblueing of organic dyes can cause artifacts in super-resolution microscopy

Commonly used organic dyes can photoconvert to blue-shifted fluorescent species, especially under intense illumination. The mechanism of this process and how to avoid unwanted artifacts in super-resolution microscopy are explored here.

Dominic A. Helmerich
Gerti Beliu
Markus Sauer
Research25 Feb 2021
Nature Methods

Volume: 18, P: 253-257
Trifunctional sphingomyelin derivatives enable nanoscale resolution of sphingomyelin turnover in physiological and infection processes via expansion microscopy

The enzymatic breakdown of the lipid sphingomyelin is associated with various infectious diseases. Here, the authors introduce trifunctional sphingomyelin derivatives for the visualisation of sphingomyelin distribution and sphingomyelinase activity in infection processes.

Marcel Rühling
Louise Kersting
Jürgen Seibel
ResearchOpen Access28 Aug 2024
Nature Communications

Volume: 15, P: 1-16
Cooperation of local motions in the Hsp90 molecular chaperone ATPase mechanism

Monitoring conformational switching within the chaperone Hsp90 using high-resolution fluorescent probes based on photoinduced electron transfer defines a cooperativity mechanism as well as a two-step restructuring of the ATP lid segment.

Andrea Schulze
Gerti Beliu
Hannes Neuweiler
Research20 Jun 2016
Nature Chemical Biology

Volume: 12, P: 628-635
Approach to map nanotopography of cell surface receptors

A super-resolution localisation-based method is shown to map receptor topography in immune cells, which allows quantitative study of molecules and their interactions at the complex surface nanomorphology of cells.

Christian Franke
Tomáš Chum
Marek Cebecauer
ResearchOpen Access09 Mar 2022
Communications Biology

Volume: 5, P: 1-16

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Photoswitching fingerprint analysis bypasses the 10-nm resolution barrier

Photoblueing of organic dyes can cause artifacts in super-resolution microscopy

Trifunctional sphingomyelin derivatives enable nanoscale resolution of sphingomyelin turnover in physiological and infection processes via expansion microscopy

Cooperation of local motions in the Hsp90 molecular chaperone ATPase mechanism

Approach to map nanotopography of cell surface receptors

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