Optical frequency domain imaging (OFDI) is a wide-field, three-dimensional intravital imaging technique that provides information on the entire tumor vasculature and surrounding tissue microenvironment, allowing visualization of angiogenesis and lymphangiogenesis during tumor growth and with therapy. Here, Vakoc et al. show that, in contrast to multiphoton microscopy, OFDI can image at greater tissue depths with a wider field of view and without the need for exogenous contrast agents.
- Benjamin J Vakoc
- Ryan M Lanning
- Brett E Bouma
