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Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex

Volume EM provides connectomic data but lacks necessary molecular information. Here, authors show fluorescent scFvs enable multiplexed immunolabeling and correlated light and electron microscopy, suggesting potential for molecularly annotated connectomic data.

Xiaomeng Han
Xiaotang Lu
Jeff W. Lichtman
ResearchOpen Access05 Aug 2024
Nature Communications

Volume: 15, P: 1-18

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Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex

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