The authors examined the feasibility of co-staining miRNAs by fluorescent miRNA in situ hybridization (miRisH) and their target proteins by immunohistofluorescence (IHF) on tissue microarrays from prostate cancer patients, which would allow for the study of miRNA expression patterns and their target proteins at the single-cell level. The miRNAs and corresponding target proteins include the pairs miR-145/ERG, miR-143/uPAR and miR-375/SEC23A.
- Markus Eckstein
- Verena Sailer
- Sven Wach