Advanced search

Filter By:

Journal Check one or more journals to show results from those journals only.

Choose more journals

Article type Check one or more article types to show results from those article types only.
Subject Check one or more subjects to show results from those subjects only.
Date Choose a date option to show results from those dates only.

Custom date range

Clear all filters
Sort by:
Showing 1–18 of 18 results
Advanced filters: Author: Emily LeProust Clear advanced filters

  • Nature Biotechnology asks a selection of leaders from across biotech to look at the future of the sector and make some predictions for the coming years.

    • Katrine Bosley
    • Charlotte Casebourn
    • Bowen Zhao
    News
    Nature Biotechnology
    Volume: 39, P: 654-660

  • On-array synthesis of over 20,000 shRNAs at a coverage of ∼30 shRNAs per gene, followed by cloning into lentiviral shRNA libraries and deconvolution of the complex libraries by deep sequencing, ensures high confidence in the observed knockdown phenotypes with low false-negative rates and few off-target hits.

    • Michael C Bassik
    • Robert Jan Lebbink
    • Michael T McManus
    Research
    Nature Methods
    Volume: 6, P: 443-445

  • An efficient and scalable strategy with robust error correction is reported for encoding a record amount of information (including images, text and audio files) in DNA strands; a ‘DNA archive’ has been synthesized, shipped from the USA to Germany, sequenced and the information read.

    • Nick Goldman
    • Paul Bertone
    • Ewan Birney
    Research
    Nature
    Volume: 494, P: 77-80

  • Padlock probes, synthesized in large scale on programmable microarrays, capture expressed single-nucleotide polymorphisms for high-throughput sequencing in this method for RNA allelotyping. The approach combines the sensitivity of digital expression measurements with the efficiency of targeted resequencing to quantify allele specific gene expression in various tissues across several individuals.

    • Kun Zhang
    • Jin Billy Li
    • George M Church
    Research
    Nature Methods
    Volume: 6, P: 613-618

  • The human cytomegalovirus protein US11 downregulates host immune responses by redirecting HLA class I molecules for endoplasmic reticulum-associated protein degradation. Using a high-coverage genome-wide shRNA screen, the authors identify TMEM129 as an E3 ubiquitin ligase essential for this process.

    • Michael L. van de Weijer
    • Michael C. Bassik
    • Robert Jan Lebbink
    ResearchOpen Access
    Nature Communications
    Volume: 5, P: 1-14

  • Although technically feasible, whole-genome analysis of cytosine methylation using bisulfite sequencing remains prohibitively expensive for large eukaryotic genomes. Deng et al. use 30,000 nondegenerate padlock probes to capture ∼66,000 bisulfite-converted sites in human CpG islands and compare their methylation in fibroblasts, embryonic stem cells and induced pluripotent stem cells.

    • Jie Deng
    • Robert Shoemaker
    • Kun Zhang
    Research
    Nature Biotechnology
    Volume: 27, P: 353-360

  • Larman et al. create a phage library containing >400,000 sequences encoding peptides that cover all open reading frames in the human genome. They then use this synthetic peptidome to discover novel autoantigens targeted by antibodies in the cerebrospinal fluid of individuals with a neurological autoimmune disease.

    • H Benjamin Larman
    • Zhenming Zhao
    • Stephen J Elledge
    Research
    Nature Biotechnology
    Volume: 29, P: 535-541

  • Nicholas Luscombe, Cameron Osborne and colleagues report the use of Capture Hi-C (CHi-C) to detect the long-range interactions of almost 22,000 promoters in 2 human cell types. They found that transcriptionally inactive genes interact with previously uncharacterized elements that may act as long-range silencers.

    • Borbala Mifsud
    • Filipe Tavares-Cadete
    • Cameron S Osborne
    Research
    Nature Genetics
    Volume: 47, P: 598-606

  • Sarah Elderkin and colleagues show that PRC1 acts as a master regulator of genome architecture in mouse embryonic stem cells by organizing genes in three-dimensional interaction networks. They find that the strongest spatial network is composed of the four Hox clusters and key early developmental transcription factor genes, and they propose that selective release of genes from this spatial network underlies cell fate specification during embryonic development.

    • Stefan Schoenfelder
    • Robert Sugar
    • Sarah Elderkin
    Research
    Nature Genetics
    Volume: 47, P: 1179-1186

  • Long DNA molecules, such as those encoding genes, can be assembled from short oligonucleotides created on a microarray. Kosuri et al. improve the fidelity and scalability of this process, enabling synthesis of 40 antibody fragments having repetitive regions and other challenging sequence features.

    • Sriram Kosuri
    • Nikolai Eroshenko
    • George M Church
    Research
    Nature Biotechnology
    Volume: 28, P: 1295-1299

  • This study tests the importance of the intrinsic DNA sequence preferences of nucleosomes by measuring the genome-wide occupancy of nucleosomes assembled on purified yeast genomic DNA. The resulting map is similar to in vivo nucleosome maps, indicating that the organization of nucleosomes in vivo is largely governed by the underlying genomic DNA sequence.

    • Noam Kaplan
    • Irene K. Moore
    • Eran Segal
    ResearchOpen Access
    Nature
    Volume: 458, P: 362-366