Nature Search

In situ secondary structure imaging of protein phase separation and aggregation by hyperspectral stimulated Raman scattering microscopy

Protein secondary structures are visualized in situ by hyperspectral stimulated Raman scattering microscopy, revealing disordered to ordered changes in protein phase separation and structural heterogeneity of subcellular aggregates in live cells.

Ruifeng Sun
Yanrong Zhuang
Fanghao Hu
ResearchOpen Access29 Sept 2025
Nature Communications

Volume: 16, P: 1-13
Author Correction: Ultra-bright Raman dots for multiplexed optical imaging

Zhilun Zhao
Chen Chen
Wei Min
Amendments and CorrectionsOpen Access01 Apr 2021
Nature Communications

Volume: 12, P: 1
Supermultiplexed optical imaging and barcoding with engineered polyynes

A class of probes enables highly multiplexed imaging and barcoding applications with Raman microscopy.

Fanghao Hu
Chen Zeng
Wei Min
Research15 Jan 2018
Nature Methods

Volume: 15, P: 194-200
Phenazine production promotes antibiotic tolerance and metabolic heterogeneity in Pseudomonas aeruginosa biofilms

Pseudomonas aeruginosa releases redox-active metabolites called phenazines. Here, the authors use metabolic imaging by stimulated Raman scattering microscopy to show that phenazines antagonize the effects of antibiotics on P. aeruginosa biofilms by modulating bacterial metabolism.

Konstanze T. Schiessl
Fanghao Hu
Lars E. P. Dietrich
ResearchOpen Access15 Feb 2019
Nature Communications

Volume: 10, P: 1-10
Photoswitchable polyynes for multiplexed stimulated Raman scattering microscopy with reversible light control

Bioimaging with photocontrol and multiplexing capability is vital for studying cellular interactions and dynamics, but multiplexed stimulated Raman scattering (SRS) imaging with reversible photocontrol is elusive. Here, the authors report SRS microscopy with Carbow-switch enabling multiplexed SRS imaging and tracking in live cells with reversible photocontrol and high spatiotemporal selectivity.

Yueli Yang
Xueyang Bai
Fanghao Hu
ResearchOpen Access22 Mar 2024
Nature Communications

Volume: 15, P: 1-12
Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering

The combination of stimulated Raman scattering (SRS) microscopy with an alkyne group as a Raman-active tag allows high-contrast, live-cell dynamic imaging of a variety of biomolecules including DNA, RNA, protein, lipids and small-molecule drugs.

Lu Wei
Fanghao Hu
Wei Min
Research02 Mar 2014
Nature Methods

Volume: 11, P: 410-412
EphrinB2-mediated CDK5/ISL1 pathway enhances cardiac lymphangiogenesis and alleviates ischemic injury by resolving post-MI inflammation

Yingnan Bai
Liming Chen
Junbo Ge
ResearchOpen Access18 Nov 2024
Signal Transduction and Targeted Therapy

Volume: 9, P: 1-15
Ultra-bright Raman dots for multiplexed optical imaging

Raman-based imaging of biomarkers is often challenging due to low sensitivity. Here, the authors use a swelling-diffusion approach to develop a series of Raman probes that are both ultra-bright and compact in size, and demonstrate multiplexed imaging of specific protein targets in cells and tissue slices.

Zhilun Zhao
Chen Chen
Wei Min
ResearchOpen Access26 Feb 2021
Nature Communications

Volume: 12, P: 1-12
9-Cyanopyronin probe palette for super-multiplexed vibrational imaging

The authors develop a method to build Manhattan Raman Scattering (MARS) probes based on different core atoms, conjugation ring numbers, and stable isotope substitutions. A quantitative model predicts vibrational frequencies of MARS dyes from structures, which are used in supermultiplexed vibrational imaging.

Yupeng Miao
Naixin Qian
Wei Min
ResearchOpen Access26 Jul 2021
Nature Communications

Volume: 12, P: 1-10
Multiplexed live-cell profiling with Raman probes

Currently relatively few functional probes for Raman-based live-cell profiling exist. Here the authors build on their previous ultra-bright Raman dots to devise a 14-plexed Raman probe panel to quantify cell surface proteins, endocytosis activities and metabolic dynamics of single live cells.

Chen Chen
Zhilun Zhao
Wei Min
ResearchOpen Access07 Jun 2021
Nature Communications

Volume: 12, P: 1-13
Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy

Recent studies have proposed that intracellular water is different from bulk water at the molecular level. Here the authors introduce a Raman microscopy-based method to image the distribution of water states in living cells, and report intracellular water heterogeneity between nucleus and cytoplasm.

Lixue Shi
Fanghao Hu
Wei Min
ResearchOpen Access18 Oct 2019
Nature Communications

Volume: 10, P: 1-8
Identification of essential sites of lipid peroxidation in ferroptosis

Ferroptosis is a lipid-peroxide-driven cell death with promising therapeutic applications. Although peroxidation of various subcellular membranes can initiate ferroptosis, the authors found that the endoplasmic reticulum is an essential site.

A. Nikolai von Krusenstiern
Ryan N. Robson
Brent R. Stockwell
Research06 Feb 2023
Nature Chemical Biology

Volume: 19, P: 719-730
Illuminating life processes by vibrational probes

This Review introduces sensitive and multiplexable chemical probes that have transformed vibrational imaging methods such as Raman microscopy beyond label-free imaging.

Naixin Qian
Zhilun Zhao
Wei Min
Reviews13 May 2025
Nature Methods

Volume: 22, P: 928-944
Biological imaging of chemical bonds by stimulated Raman scattering microscopy

This Perspective summarizes advances in stimulated Raman spectroscopy (SRS) instruments and probes, and highlights biological discoveries made with these technologies.

Fanghao Hu
Lixue Shi
Wei Min
Reviews30 Aug 2019
Nature Methods

Volume: 16, P: 830-842
Super-multiplex vibrational imaging

Stimulated Raman scattering under electronic pre-resonance conditions, combined with a new palette of probes, enables super-multiplex imaging of molecular targets in living cells with very high vibrational selectivity and sensitivity.

Lu Wei
Zhixing Chen
Wei Min
Research19 Apr 2017
Nature

Volume: 544, P: 465-470
Bioorthogonal chemical imaging of metabolic activities in live mammalian hippocampal tissues with stimulated Raman scattering

Fanghao Hu
Michael R. Lamprecht
Wei Min
ResearchOpen Access21 Dec 2016
Scientific Reports

Volume: 6, P: 1-11

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In situ secondary structure imaging of protein phase separation and aggregation by hyperspectral stimulated Raman scattering microscopy

Author Correction: Ultra-bright Raman dots for multiplexed optical imaging

Supermultiplexed optical imaging and barcoding with engineered polyynes

Phenazine production promotes antibiotic tolerance and metabolic heterogeneity in Pseudomonas aeruginosa biofilms

Photoswitchable polyynes for multiplexed stimulated Raman scattering microscopy with reversible light control

Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering

EphrinB2-mediated CDK5/ISL1 pathway enhances cardiac lymphangiogenesis and alleviates ischemic injury by resolving post-MI inflammation

Ultra-bright Raman dots for multiplexed optical imaging

9-Cyanopyronin probe palette for super-multiplexed vibrational imaging

Multiplexed live-cell profiling with Raman probes

Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy

Identification of essential sites of lipid peroxidation in ferroptosis

Illuminating life processes by vibrational probes

Biological imaging of chemical bonds by stimulated Raman scattering microscopy

Super-multiplex vibrational imaging

Bioorthogonal chemical imaging of metabolic activities in live mammalian hippocampal tissues with stimulated Raman scattering

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