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Showing 1–5 of 5 results
Advanced filters: Author: Florian Heigwer Clear advanced filters
  • CRISPR gene targeting in multicellular organisms results in genetic mosaics, limiting knockout efficiency. Here, the authors develop an improved system using Cas12a with multiple guides per gene, and demonstrate high accuracy and superior knockout efficiency in fruit flies.

    • Fillip Port
    • Martha A. Buhmann
    • Michael Boutros
    ResearchOpen Access
    Nature Communications
    Volume: 17, P: 1-16
  • Microscopy-based drug screens with fluorescent markers can shed light on how drugs affect biological processes. Without adding markers and imaging channels, which is cumbersome and costly, a new generative deep-learning method extracts new fluorescence channels from images, potentially improving the drug-discovery pipeline.

    • Florian Heigwer
    News & Views
    Nature Machine Intelligence
    Volume: 4, P: 616-617
  • This Review covers the steps required to create high-quality image-based profiles from high-throughput microscopy images.

    • Juan C Caicedo
    • Sam Cooper
    • Anne E Carpenter
    ReviewsOpen Access
    Nature Methods
    Volume: 14, P: 849-863
  • Neufeldt et al. evaluate transcriptional and cytokine secretion profiles of cells and patient sera following SARS-CoV-2 infection and detect distinct upregulation of inflammatory cytokines. They also demonstrate that this upregulation is mediated by cGAS-STING and NF-κB signalling, which could provide a potential avenue for the development of future therapies against SARS-CoV-2.

    • Christopher J. Neufeldt
    • Berati Cerikan
    • Ralf Bartenschlager
    ResearchOpen Access
    Communications Biology
    Volume: 5, P: 1-15