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Showing 1–11 of 11 results
Advanced filters: Author: Floyd E Romesberg Clear advanced filters

  • Triphosphates of hydrophobic nucleotides d5SICS and dNaM are imported into Escherichia coli by an exogenous algal nucleotide triphosphate transporter and then used by an endogenous polymerase to replicate, and faithfully maintain over many generations of growth, a plasmid containing the d5SICS–dNaM unnatural base pair.

    • Denis A. Malyshev
    • Kirandeep Dhami
    • Floyd E. Romesberg
    Research
    Nature
    Volume: 509, P: 385-388

  • Many efforts to expand the genetic alphabet and reprogram the genetic code have relied on synthetic DNA nucleotides designed to have pairing properties orthogonal to those of natural base pairs. A structural study shows that DNA polymerases enhance the efficiency of non-natural base pair replication by enforcing a standard Watson-Crick geometry in the polymerase active site.

    • Karin Betz
    • Denis A Malyshev
    • Andreas Marx
    Research
    Nature Chemical Biology
    Volume: 8, P: 612-614

  • Naturally occurring DNA polymerases can amplify DNA efficiently via PCR, but they cannot utilize C2′-modified substrates to make non-natural nucleic acids. Such C2′-modified nucleic acids are of interest as they are resistant to nucleases. Now, a Stoffel fragment DNA polymerase has been evolved to transcribe C2′-modified DNA from a DNA template, reverse transcribe C2′-modified DNA back into DNA, and PCR-amplify C2′-modified DNA.

    • Tingjian Chen
    • Narupat Hongdilokkul
    • Floyd E. Romesberg
    Research
    Nature Chemistry
    Volume: 8, P: 556-562

  • Systematic characterization of codons using the unnatural base pair dNaM·dTPT3 leads to the discovery of nine new functional codon–anticodon pairs, three of which are shown to be orthogonally decoded by ribosomes and allow incorporation of up to three noncanonical amino acids in Escherichia coli.

    • Emil C. Fischer
    • Koji Hashimoto
    • Floyd E. Romesberg
    Research
    Nature Chemical Biology
    Volume: 16, P: 570-576

  • DNA containing a new unnatural base pair may be amplified by PCR and transcribed into RNA, potentially increasing the diversity available from nucleic acids.

    • Aaron M Leconte
    • Floyd E Romesberg
    News & Views
    Nature Methods
    Volume: 3, P: 667-668

  • Slipping in extra benzene rings creates a broader DNA double helix that is similar to, but different from, natural DNA. Importantly, it can encode more genetic information — and that could have wide implications.

    • Aaron M. Leconte
    • Floyd E. Romesberg
    News & Views
    Nature
    Volume: 444, P: 553-555

  • Certain antibiotics prompt Streptococcus pneumoniae to take up DNA from the environment, which may enable the microbe to acquire genes for antibiotic resistance.

    • Ryan T Cirz
    • Neill Gingles
    • Floyd E Romesberg
    News & Views
    Nature Medicine
    Volume: 12, P: 890-891