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Showing 1–6 of 6 results
Advanced filters: Author: Francesca Bottanelli Clear advanced filters

  • Tracking fast molecules in crowded organelles is error-prone, obscuring dynamic processes like Alzheimer’s secretase activity or secretory sorting. Here, authors present FidlTrack, a structure-aware method that boosts tracking fidelity and resolves ER-exit, nanobody binding, and BACE1-APP cleavage.

    • Pierre Parutto
    • Yutong Yuan
    • Edward Avezov
    ResearchOpen Access
    Nature Communications
    P: 1-24

  • The intracellular applications of STED microscopy are limited by the availability of dyes. Here the authors develop a two-colour labelling strategy based on SiR and ATTO590 dyes, and apply their strategy to image various subcellular membrane compartments.

    • Francesca Bottanelli
    • Emil B. Kromann
    • Joerg Bewersdorf
    ResearchOpen Access
    Nature Communications
    Volume: 7, P: 1-5

  • Stockhammer, Adarska et al. describe ARF1 compartments as the site of adaptor- and clathrin-dependent post-Golgi sorting. Shedding of ARF1 and maturation into recycling endosomes drives sorting of secretory and endocytic recycling cargo.

    • Alexander Stockhammer
    • Petia Adarska
    • Francesca Bottanelli
    ResearchOpen Access
    Nature Cell Biology
    Volume: 26, P: 1845-1859

  • Stimulated emission depletion microscopy opens new observation windows in challenging samples such as living cells and tissues. In this Primer, Lukinavičius et al. discuss 2D and 3D stimulated emission depletion setup, including adaptive optical elements and their combination with fluorescence lifetime techniques.

    • Gražvydas Lukinavičius
    • Jonatan Alvelid
    • Ilaria Testa
    Reviews
    Nature Reviews Methods Primers
    Volume: 4, P: 1-22