HaloPlexHS target enrichment incorporates molecular barcodes in hybridization probes in order to detect data resulting from PCR errors. The authors combined HaloPlexHS technology with Illumina HiSeq sequencing and developed a new validated variant-calling algorithm. Thus, they were able to establish a targeted sequencing protocol that allows reliable mutation detection with a single replicate using DNA samples from formalin-fixed paraffin-embedded tissues.
- Francesco Cucco
- Alexandra Clipson
- Ming-Qing Du
