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Showing 1–10 of 10 results
Advanced filters: Author: Gaelen T. Hess Clear advanced filters

  • Recruiting a hyperactive cytidine deaminase via the guide RNA to dCas9 allows for the introduction of diverse point mutations at the CRISPR target locus to create complex libraries of variants for protein engineering or dissection of protein function.

    • Gaelen T Hess
    • Laure Frésard
    • Michael C Bassik
    Research
    Nature Methods
    Volume: 13, P: 1036-1042

  • CRISPR-Cas9 screens are powerful high-throughput tools but can be confounded by nuclease toxicity. Here the authors design a library of variable length gRNAs with thousands of negative controls, including the targeting of ‘safe’ loci to account for on-target site DNA damage toxicity.

    • David W. Morgens
    • Michael Wainberg
    • Michael C. Bassik
    ResearchOpen Access
    Nature Communications
    Volume: 8, P: 1-8

  • Off-target effects in CRISPR screens for essential regulatory elements have not been systematically evaluated. Here the authors find Cas9 nuclease, CRISPRi/a each have distinct off-target effects, and that these can be accurately identified and removed using the GuideScan sgRNA specificity score.

    • Josh Tycko
    • Michael Wainberg
    • Michael C. Bassik
    ResearchOpen Access
    Nature Communications
    Volume: 10, P: 1-14

  • CRISPR base- and prime-editing pooled screens reveal the function of genetic variants at unprecedented resolution.

    • Peter P. Du
    • Katherine Liu
    • Gaelen T. Hess
    News & Views
    Nature Biotechnology
    Volume: 40, P: 834-836

  • Francesco Cucca, Stephen Montgomery and colleagues identify regulatory variants that influence gene expression and splicing using whole-genome and transcriptome sequence data from 624 Sardinians. They find a high frequency of splicing and expression quantitative trait loci near genes involved in malarial resistance and multiple sclerosis.

    • Mauro Pala
    • Zachary Zappala
    • Stephen B Montgomery
    Research
    Nature Genetics
    Volume: 49, P: 700-707

  • The authors show that rare genetic variants contribute to large gene expression changes across diverse human tissues and provide an integrative method for interpretation of rare variants in individual genomes.

    • Xin Li
    • Yungil Kim
    • Stephen B. Montgomery
    ResearchOpen Access
    Nature
    Volume: 550, P: 239-243

  • Translation termination sequences are occasionally bypassed by the ribosome and the resulting proteins can be detrimental to the cell; here it is shown that cells can prevent such proteins from accumulating through peptides that are encoded within the 3' UTR of genes in both humans and C. elegans.

    • Joshua A. Arribere
    • Elif S. Cenik
    • Andrew Z. Fire
    Research
    Nature
    Volume: 534, P: 719-723