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Showing 1–7 of 7 results
Advanced filters: Author: George Wadhams Clear advanced filters

  • The dynamic exchange of motor proteins in a functioning 45-nm rotary complex was observed in vivo and GFP–MotB stator proteins visualized in individual bacterial flagellar motors. Fluorescence recovery after photobleaching reveals that the ∼22 MotB motor proteins exchange rapidly with a pool of free protein while the motor is actively rotating.

    • Mark C. Leake
    • Jennifer H. Chandler
    • Judith P. Armitage
    Research
    Nature
    Volume: 443, P: 355-358

  • In this Review, Armitage and colleagues describe how some bacterial species, as typified byRhodobacter sphaeroides, have evolved to contain complex chemotaxis signalling networks that integrate sensory information from the environment with metabolic information from within the cell to produce a balanced response at the flagellar motor.

    • Steven L. Porter
    • George H. Wadhams
    • Judith P. Armitage
    Reviews
    Nature Reviews Microbiology
    Volume: 9, P: 153-165

  • In an inter-laboratory study, the authors compare the accuracy and performance of three optical density calibration protocols (colloidal silica, serial dilution of silica microspheres, and colony-forming unit (CFU) assay). They demonstrate that serial dilution of silica microspheres is the best of these tested protocols, allowing precise and robust calibration that is easily assessed for quality control and can also evaluate the effective linear range of an instrument.

    • Jacob Beal
    • Natalie G. Farny
    • Jiajie Zhou
    ResearchOpen Access
    Communications Biology
    Volume: 3, P: 1-29