Saturation transfer difference (STD) NMR analysis was performed to identify the titanium-binding peptide (TBP) sites that interact with the SiO2 nanoparticle surface, and then Arg1 and Asp5 were identified to be in close contact with the surface. The structure of the TBP bound to SiO2 determined by the NOESY measurement was well defined, and the Arg1 and Asp5 side chains face in the same direction. These results validates that the NH2+ of Arg1 and the COO− of Asp5 interact electrostatically with the SiO− and SiOH2+ on the SiO2 surface, respectively.
- Yu Suzuki
- Heisaburo Shindo
