Tumor-infiltrating lymphocyte (TIL) therapy has demonstrated clinical benefit in patients with solid tumors. Here the authors propose a protocol for isolating and enriching TILs from cavitron ultrasonic aspirator emulsions of patients with glioma, showing the successful expansion of functional tumor-reactive TILs for personalized cell therapy.
- Martina Maffezzini
- Silvia Musio
- Serena Pellegatta
