Using small-angle X-ray scattering, Hajdú et al. show that Rho-associated protein kinase 2 population is a mixture of folded and partially extended conformers. They find that the binding of natural protein substrates to the kinase domain breaks up the interaction between the N-terminal kinase and C-terminal regulatory domains. This study identifies a dynamic behavior of this long, dimeric molecule in solution.
- István Hajdú
- András Szilágyi
- Péter Závodszky
