Mur ligase D (MurD) is a promising antibacterial target, but its dynamics remain underexplored. Here, the authors use 15N nuclear magnetic resonance to probe apo and bound states of MurD in solution, showing that dynamic events on a time scale of picoseconds to milliseconds have to be considered in the design of structurally novel Mur inhibitors.
- Iza Ogris
- Barbara Zupančič
- Simona Golič Grdadolnik
