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Showing 1–2 of 2 results
Advanced filters: Author: Izabela Plumbom Clear advanced filters

  • T-cell–mediated rejection (TCMR) remains a major cause of kidney transplant failure with incompletely understood mechanisms. Here the authors use single-nucleus RNA sequencing, spatial transcriptomics and immunofluorescence to show that injured kidney epithelial cell states associate with poor transplant outcomes after T-cell–mediated rejection.

    • Anna Maria Pfefferkorn
    • Lorenz Jahn
    • Christian Hinze
    ResearchOpen Access
    Nature Communications
    Volume: 17, P: 1-19

  • Thomas Conrad et al. identify sequences downstream of transcription start sites that affect T7 promoter activity and use this to generate promoter variants with enhanced transcriptional output. They use the optimized promoters to prepare cDNA libraries for single-cell RNA sequencing with increased complexity compared to those prepared with standard T7 promoters.

    • Thomas Conrad
    • Izabela Plumbom
    • Sascha Sauer
    ResearchOpen Access
    Communications Biology
    Volume: 3, P: 1-8