Monitoring glycosylation is a key quality control for protein therapeutics, however, most established methods such as hydrophilic interaction chromatography with fluorescence detection (HILIC-Fld) and liquid chromatography-mass spectrometry (LC-MS) require time-consuming procedures and complicated data analysis. Here, the authors apply high performance anion exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD) for the batch-to-batch monitoring of glycans on complex antigens with a simplified procedure.
- Janelle Sauvageau
- Izel Koyuturk
- Yves Durocher
