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A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells

A new riboswitch-based RNA sensor called Riboglow binds to quenched fluorescent probes to induce fluorescence turn-on. Riboglow enables tagging and tracking of mRNA and short noncoding RNAs with different colored fluorophores in live mammalian cells.

Esther Braselmann
Aleksandra J. Wierzba
Amy E. Palmer
Research30 Jul 2018
Nature Chemical Biology

Volume: 14, P: 964-971
Minor intron retention drives clonal hematopoietic disorders and diverse cancer predisposition

Loss of function of the minor spliceosome component ZRSR2 enhances hematopoietic stem cell self-renewal through minor intron retention of its target LZTR1, which is a regulator of RAS-related GTPases. Minor intron retention of LZTR1 was also identified in Noonan syndrome and diverse solid tumor types.

Daichi Inoue
Jacob T. Polaski
Omar Abdel-Wahab
Research12 Apr 2021
Nature Genetics

Volume: 53, P: 707-718
RNA isoform screens uncover the essentiality and tumor-suppressor activity of ultraconserved poison exons

pgFARM (paired guide RNAs for alternative exon removal) is a CRISPR–Cas9-based approach to manipulate alternative splicing and identify functional roles for individual exons, including poison exons with essential and tumor-suppressor roles.

James D. Thomas
Jacob T. Polaski
Robert K. Bradley
Research07 Jan 2020
Nature Genetics

Volume: 52, P: 84-94
B₁₂ cofactors directly stabilize an mRNA regulatory switch

The crystal structures of two different cobalamin (vitamin B₁₂)-binding riboswitches are determined; the structures reveal how cobalamin facilitates interdomain interactions to regulate gene expression.

James E. Johnson Jr
Francis E. Reyes
Robert T. Batey
Research14 Oct 2012
Nature

Volume: 492, P: 133-137
Synthetic introns enable splicing factor mutation-dependent targeting of cancer cells

Synthetic introns tailored for specific splice-factor mutations enable targeted cancer gene therapy.

Khrystyna North
Salima Benbarche
Robert K. Bradley
Research03 Mar 2022
Nature Biotechnology

Volume: 40, P: 1103-1113
Recurrent RNA motifs as scaffolds for genetically encodable small-molecule biosensors

In vitro selection of RNA libraries constructed by randomization of RNA structural scaffolds from known ribozymes and riboswitches led to the identification of aptamers that were readily translated into functional biosensors in cells.

Ely B Porter
Jacob T Polaski
Robert T Batey
Research16 Jan 2017
Nature Chemical Biology

Volume: 13, P: 295-301
Characterization of HCI-EC-23 a novel estrogen- and progesterone-responsive endometrial cancer cell line

Craig M. Rush
Zannel Blanchard
Jason Gertz
ResearchOpen Access17 Nov 2022
Scientific Reports

Volume: 12, P: 1-17

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A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells

Minor intron retention drives clonal hematopoietic disorders and diverse cancer predisposition

RNA isoform screens uncover the essentiality and tumor-suppressor activity of ultraconserved poison exons

B12 cofactors directly stabilize an mRNA regulatory switch

Synthetic introns enable splicing factor mutation-dependent targeting of cancer cells

Recurrent RNA motifs as scaffolds for genetically encodable small-molecule biosensors

Characterization of HCI-EC-23 a novel estrogen- and progesterone-responsive endometrial cancer cell line

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B₁₂ cofactors directly stabilize an mRNA regulatory switch