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Simultaneous and sensitive quantification of protein and low molecular weight persulfides, polysulfides and H₂S in biological samples

Hydrogen sulfide (H₂S) reversibly modifies low molecular weight and protein thiols to form persulfides (RSS⁻) and polysulfides (RS(S)nS⁻) for antioxidant defence and regulation of activity. Here, the authors report a sensitive LC-MS/MS procedure that separately traps and quantifies the sulfur atom of H₂S, the terminal sulfur atom of RSS and RS(S)nS-, and the internal sulfur atoms of RS(S)nS ⁻ as diagnostic products in biological samples.

Jan Lj. Miljkovic
Nils Burger
Michael P. Murphy
ResearchOpen Access04 Dec 2025
Nature Communications

Volume: 17, P: 1-19
Naked mole-rats have distinctive cardiometabolic and genetic adaptations to their underground low-oxygen lifestyles

The naked mole-rat exhibits extreme longevity, resistance to hypoxia and absence of cardiovascular disease. Here, Faulkes et al. identify mechanisms behind these traits by comparing cardiac metabolomes and transcriptomes of naked more-rats to other African mole-rat genera and evolutionary divergent mammals.

Chris G. Faulkes
Thomas R. Eykyn
Dunja Aksentijevic
ResearchOpen Access27 Mar 2024
Nature Communications

Volume: 15, P: 1-13
Pro-inflammatory macrophages produce mitochondria-derived superoxide by reverse electron transport at complex I that regulates IL-1β release during NLRP3 inflammasome activation

Casey et al. explore the basic mechanisms of mitochondrial superoxide production and its impact on interleukin-1β production in LPS-treated macrophages.

Alva M. Casey
Dylan G. Ryan
Michael P. Murphy
ResearchOpen Access19 Feb 2025
Nature Metabolism

Volume: 7, P: 493-507

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Simultaneous and sensitive quantification of protein and low molecular weight persulfides, polysulfides and H2S in biological samples

Naked mole-rats have distinctive cardiometabolic and genetic adaptations to their underground low-oxygen lifestyles

Pro-inflammatory macrophages produce mitochondria-derived superoxide by reverse electron transport at complex I that regulates IL-1β release during NLRP3 inflammasome activation

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Simultaneous and sensitive quantification of protein and low molecular weight persulfides, polysulfides and H₂S in biological samples