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Showing 1–2 of 2 results
Advanced filters: Author: Jayesh A. Bafna Clear advanced filters
  • In vitro protein folding can often result in aggregation and low yields. Here the authors use nanoscale exoshells to improve soluble yield, functional yield and specific activity of folded proteins.

    • Samira Sadeghi
    • Siddharth Deshpande
    • Chester L. Drum
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-15
  • Bacillus thuringiensis israelensis (Bti) produces the naturally-crystalline proteinaceous toxin Cyt1Aa that is toxic to mosquito larvae. Here the authors grow recombinant nanocrystals of the Cyt1Aa protoxin in vivo and use serial femtosecond crystallography to determine its structure at different redox and pH conditions and by combining their structural data with further biochemical, toxicological and biophysical analyses provide mechanistic insights into the Cyt1Aa bioactivation cascade.

    • Guillaume Tetreau
    • Anne-Sophie Banneville
    • Jacques-Philippe Colletier
    ResearchOpen Access
    Nature Communications
    Volume: 11, P: 1-16