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Showing 1–5 of 5 results
Advanced filters: Author: Jesse G. Zalatan Clear advanced filters
  • Guide RNA folding affects functionality of CRISPR-Cas transcriptional control systems. Here, the authors report computational gRNA design together with creation of synthetic CRISPRa promoters for orthogonal expression control and demonstrate the application in pteridine and human milk oligosaccharide production in E. coli.

    • Jason Fontana
    • David Sparkman-Yager
    • James M. Carothers
    ResearchOpen Access
    Nature Communications
    Volume: 15, P: 1-16
  • The absence of effective gene activators in bacteria limits regulated expression programs. Here the authors design synthetic bacterial CRISPR-Cas transcriptional activators that can be used to construct multi-gene programs of activation and repression.

    • Chen Dong
    • Jason Fontana
    • Jesse G. Zalatan
    ResearchOpen Access
    Nature Communications
    Volume: 9, P: 1-11
  • The scope of enzymology has expanded rapidly over the last century, from an early focus on the chemical and catalytic mechanisms of individual enzymes to more recent efforts to understand enzyme action in the context of dynamic, functional biological systems consisting of many interacting enzymes and proteins. Continuing progress in probing the link between molecular structure and function now promises to pave the way for a deeper understanding of the evolution and behavior of the complex biological systems that govern cellular behavior.

    • Jesse G Zalatan
    • Daniel Herschlag
    Comments & Opinion
    Nature Chemical Biology
    Volume: 5, P: 516-520