Cas12i, class 2 type V CRISPR-Cas system protein, uses a single RuvC domain for cleavage of both strands of target DNA. Structures of Cas12i2–crRNA–DNA complexes not only provide insight into the mechanism of DNA recognition and cleavage by Cas12i2, but also the DNA cleavage mechanism by RuvC-containing Cas proteins.
- Xue Huang
- Wei Sun
- Yanli Wang
