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Showing 1–3 of 3 results
Advanced filters: Author: Johan Oelrich Clear advanced filters

  • The use of antibodies to capture and profile exosomes limits the number of target proteins that can be detected. Here the authors develop a proximity-dependent barcoding assay that allows profiling of 38 surface proteins on individual exosomes from heterogeneous samples such as serum and seminal fluid.

    • Di Wu
    • Junhong Yan
    • Masood Kamali-Moghaddam
    ResearchOpen Access
    Nature Communications
    Volume: 10, P: 1-10

  • To improve our ability to monitor cellular responses to e.g. cytokines or drugs, Lönn et al have developed a semi-automated system for large-scale in situ proximity ligation assays (isPLA) in HaCAT keratinocyte cells. Their approach expands the scope for image-based single cell analyses by combining observations of protein interactions and modifications with morphological details of individual cells at high throughput.

    • Peter Lönn
    • Rasel A. Al-Amin
    • Ulf Landegren
    ResearchOpen Access
    Communications Biology
    Volume: 4, P: 1-9