Recent studies have indicated that a cell's proteome is significantly larger than the number of protein-coding genes due to extensive alternative splicing. This study describes an unbiased, genome-wide method to characterize RNA-protein binding interactions in vivo. The binding sites of the neuron-specific splicing factor Nova are characterized with the unexpected result that Nova may have an additional function in regulating alternative polyadenylation as well.
- Donny D. Licatalosi
- Aldo Mele
- Robert B. Darnell
