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Showing 1–2 of 2 results
Advanced filters: Author: Jongho Baek Clear advanced filters

  • Focus-locking improves localization precision in single-molecule microscopy, but fiducials are often deposited at random and provide limited 3D compensation. Here, the authors fabricate 3D optical fiducials with nanometer accuracy by two-photon direct laser writing, and demonstrate isotropic 3D focus locking.

    • Simao Coelho
    • Jongho Baek
    • Katharina Gaus
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-8

  • Mechanical sample motion (drift) is a major factor that limits fluorophore localization precision during single-molecule localization microscopy. This protocol describes how to implement 3D active stabilization onto custom and standard microscopes.

    • Simao Coelho
    • Jongho Baek
    • Katharina Gaus
    Protocols
    Nature Protocols
    Volume: 16, P: 497-515