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Showing 1–4 of 4 results
Advanced filters: Author: Julie Cramard Clear advanced filters

  • Live-cell imaging shows that interactions within topologically associating domains are transient and frequent throughout the cell cycle. Convergent CTCF sites regulate the frequency and duration of interactions, which last a few minutes on average.

    • Pia Mach
    • Pavel I. Kos
    • Luca Giorgetti
    ResearchOpen Access
    Nature Genetics
    Volume: 54, P: 1907-1918

  • The transcriptional effect of an enhancer depends on its contact probabilities with the promoter through a nonlinear relationship, and enhancer strength determines absolute transcription levels as well as the sensitivity of a promoter to CTCF-mediated transcriptional insulation.

    • Jessica Zuin
    • Gregory Roth
    • Luca Giorgetti
    ResearchOpen Access
    Nature
    Volume: 604, P: 571-577

  • A chromosome conformation capture method in which single cells are first imaged and then processed enables intact genome folding to be studied at a scale of 100 kb, validated, and analysed to generate hypotheses about 3D genomic interactions and organisation.

    • Tim J. Stevens
    • David Lando
    • Ernest D. Laue
    Research
    Nature
    Volume: 544, P: 59-64

  • Single molecule tracking of fluorescent proteins in live cells is temporally limited by fluorophore photobleaching. Here the authors show using fluorophore pairs that FRET competes with photobleaching to improve photostability and allow longer-term tracking of both single proteins and complexes.

    • Srinjan Basu
    • Lisa-Maria Needham
    • Ernest D. Laue
    ResearchOpen Access
    Nature Communications
    Volume: 9, P: 1-14